伊東研究室｜鹿児島大学理学部理学科化学プログラム生化学分野　

Professor Yuji ITO

Dear persons

I am Professor Yuji ITO, who manages this laboratory.
Our laboratory focuses on the researches toward the development of the highly functional antibody medicine. Our major technologies are phage libraries of antibody and peptide, the sites-specific conjugation method of antibody through affinity peptide, which is named CCAP (chemical conjugation by affinity peptide), and design skills of peptide. VHH and scFv. By using and combining these technologies, we are promoting many research projects supported by governmental foundations (AMED and JST) or collaborated with pharmaceutical and pharmaceutics-related companies.
If you are interested in our researches and joining to our team, we will welcome you as researchers (pos-doc or research assistant) or doctor course students. Please contact me freely.
Best wishes,

Yuji ITO (Professor, Ph. D.)
Department of Chemistry and Bioscience
Graduate School of Science and Engineering
Kagoshima University
Korimoto 1-21-35, Kagoshima, 890-0065 JAPAN
TEL/FAX: +81-99-285-8110
Skype ID: yuchan_itou
Email1: yito@sci.kagoshima-u.ac.jp
Email2: k2174603@kadai.jp
Home page: http://www.sci.kagoshima-u.ac.jp/~yito/
Project home page: http://www.i-biomed.jp/adopted-subject-a/sa06/

Publication

Paper

2021

Yudai Tabuchi, Takahito Watanabe, Riku Katsuki, Yuji Ito, Masumi Taki. "Direct screening of a target-specific covalent binder: stringent regulation of warhead reactivity at a matchmaking environment" Chemical Communications 57(2021):5378-5381.https://doi.org/10.1039/D1CC01773J.
Kyohei Muguruma, Rent Osawa, Akane Fukuda, Naoto Ishikawa, Konomi Fujita, Akihiro Taguchi, Kentaro Takayama, Atsuhiko Taniguchi, Yuji Ito, Yoshio Hayashi. "Development of a High-Affinity Antibody-Binding Peptide for Site-Specific Modification "ChemMedChem, Volume 16, Issue 11(2021):1814-1821.https://doi.org/10.1002/cmdc.202000977
Kazuto Mochizuki, Lisa Matsukura, Yuji Ito, Naoyuki Miyashita, Masumi Taki. “A Medium-Firm Drug-Candidate Library of Cryptand-like Structures on T7 Phage: Design and Selection of a Strong Binder for Hsp90” Organic and Biomolecular Chemistry 19 (2021):146-150.https://doi.org/10.1039/d0ob01855d
Satoka Mori, Arisa Abe, Naoto Ishikawa, Abdur Rafique, Yuji Ito. “A novel site-specific chemical conjugation of IgG antibodies by affinity peptide for immunoassays” The Journal of Biochemistry, Volume 169, Issue 1 (2021):35–42.https://doi.org/10.1093/jb/mvaa084.
伊東祐二, 親和性ペプチドによる部位特異的修飾ADC, BIO Clinica. 2021年6月10日発行. 東京: 株式会社北隆館, 2021.

2020

Riri Yokota, Shun Hashimoto, Ikuyo Watanabe, Satoshi Kishimoto, Masaaki Toyama, Mika Okamoto, Makoto Yoshimitsu, Kenji Ishitsuka, Yuji Ito and Masanori Baba. “Novel Anti-CD70 Antibody Drug Conjugate for the Treatment of Adult T-Cell Leukemia (ATL).” ANTICANCER RESEARCH 40 (2020):4471-4479. https://doi.org/10.21873/anticanres.14452.
Kato, Yukinari, Yuji Ito, Tomokazu Ohishi, Manabu Kawada, Takuro Nakamura, Yusuke Sayama, Masato Sano, et al. “Antibody–Drug Conjugates Using Mouse–Canine Chimeric Anti-Dog Podoplanin Antibody Exerts Antitumor Activity in a Mouse Xenograft Model.” Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 39, no. 2 (2020): 1–8. https://doi.org/10.1089/mab.2020.0001.
Wanaporn Yimchuen, Tetsuya Kadonosono, Yumi Ota, Shinichi Sato, Maika Kitazawa, Tadashi Shiozawa, Takahiro Kuchimaru, Masumi Taki, Yuji Ito, Hiroyuki Nakamura, Shinae Kizaka-Kondoh. “Strategic design to create HER2-targeting proteins with target-biding peptides immobilized on a fibronectin type Ⅲ domain scaffold.” RSC Advances Issue 26, (2020): 15154-15162. https://doi.org/10.1039/D0RA00427H.
Dai-ichiro Kato, Hirobumi Suzuki, Atsuhiro Tsuruta, Juri Maeda, Yoshinobu Hayashi, Kazunari Arima, Yuji Ito, Yukio Nagano.“Evaluation of the population structure and phylogeography of the Japanese Genji firefly, Luciola cruciata,at the nuclear DNA level using RAD-Seq analysis.”scientific reports10,(2020):1533.https://www.nature.com/articles/s41598-020-58324-9.
伊東祐二（鹿児島大学）. ヒト抗体医薬開発を目指した次世代シークエンサーとファージライブラリによる抗体設計. Edited by 植田充美（京都大学）. 細胞表層工学の進展. 2020年4月1日第1刷. 東京: 株式会社シーエムシー出版, 2020.

2019

Kishimoto, Satoshi, Yuichi Nakashimada, Riri Yokota, Takaaki Hatanaka, Motoyasu Adachi, and Yuji Ito. “Site-Specific Chemical Conjugation of Antibodies by Using Affinity Peptide for the Development of Therapeutic Antibody Format.” Research-article. Bioconjugate Chemistry 30, no. 3 (2019): 698–702. https://doi.org/10.1021/acs.bioconjchem.8b00865.
Hamano, Nobuhito, Sho Kamoshida, Yamato Kikkawa, Yusuke Yano, Tomomi Kobayashi, Endo-Takahashi. Yoko, Ryo Suzuki, et al. “Development of Antibody-Modified Nanobubbles Using Fc-Region-Binding Polypeptides for Ultrasound Imaging.” Pharmaceutics 11, no. 283 (2019): 1–10.
Yamada, Kei, Natsuki Shikida, Kazutaka Shimbo, Yuji Ito, Zahra Khedri, Yutaka Matsuda, and Brian A. Mendelsohn. “AJICAP: Affinity Peptide Mediated Regiodivergent Functionalization of Native Antibodies.” Angewandte Chemie International Edition 58, no. 17 (2019): 5592–97. https://doi.org/10.1002/anie.201814215.
Rafique, Abdur, Kiriko Satake, Satoshi Kishimoto, Kamrul Hasan Khan, Dai-ichiro Kato, and Yuji Ito. “Efficient Screening and Design of Variable Domain of Heavy Chain Antibody Ligands Through High Throughput Sequencing for Affinity Chromatography to Purify Fab Fragments.” Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 38, no. 5 (2019): 190–200. https://doi.org/10.1089/mab.2019.0027.
Muguruma, Kyohei, Konomi Fujita, Akane Fukuda, Satoshi Kishimoto, Soichiro Sakamoto, Risako Arima, Mayu Ito, et al. “Kinetics-Based Structural Requirements of Human Immunoglobulin G Binding Peptides.” Research-article. ACS Omega 4, no. 11 (2019): 14390–97. https://doi.org/10.1021/acsomega.9b01104.
Nakano, Ryosuke, Sayaka Takagi-Maeda, Yuji Ito, Satoshi Kishimoto, Tomoko Osato, Kaori Noguchi, Kana Kurihara-Suda, and Nobuaki Takahashi. “A New Technology for Increasing Therapeutic Protein Levels in the Brain over Extended Periods.” Plos One 14, no. 4 (2019): e0214404. https://doi.org/10.1371/journal.pone.0214404.
Yamada, Kei, and Yuji Ito. “Recent Chemical Approaches for Site‐Specific Conjugation of Native Antibodies: Technologies toward Next‐Generation Antibody–Drug Conjugates.” ChemBioChem 20, no. 21 (2019): 2729–37. https://doi.org/10.1002/cbic.201900178.

2018

Yuji, Ito, Kanayama Yosuke, Hayashi Yoshio, and Muguruma Kyohei. “抗体部位特異的修飾法を用いた放射性医薬品の開発.” Isotope News, no. 755 (2018): 22–24.
Muchima, Kaname, Taro Todaka, Hiroyuki Shinchi, Ayaka Sato, Arisa Tazoe, Rikiya Aramaki, Yuhei Kakitsubata, et al. “Development of Sugar Chain-Binding Single-Chain Variable Fragment Antibody to Adult T-Cell Leukemia Cells Using Glyco-Nanotechnology and Phage Display Method.” Journal of Biochemistry 163, no. 4 (April 1, 2018): 281–91. https://doi.org/10.1093/jb/mvy005.
Enomoto-Okawa, Yurie, Yuji Ito, Yamato Kikkawa, Nozomi Harashima, Yumika Sugawara, Yoichi Negishi, Motoyoshi Nomizu, et al. “Internalization of CD239 Highly Expressed in Breast Cancer Cells: A Potential Antigen for Antibody-Drug Conjugates.” Scientific Reports 8, no. 1 (2018). https://doi.org/10.1038/s41598-018-24961-4.
Uematsu, Shuta, Yudai Tabuchi, Yuji Ito, and Masumi Taki. “Combinatorially Screened Peptide as Targeted Covalent Binder: Alteration of Bait-Conjugated Peptide to Reactive Modifier.” Rapid-communication. Bioconjugate Chemistry 29, no. 6 (2018): 1866–71. https://doi.org/10.1021/acs.bioconjchem.8b00301.
Mizukami, Makoto, Hiromasa Onishi, Hiroshi Hanagata, Akira Miyauchi, Yuji Ito, Hiroko Tokunaga, Matsujiro Ishibashi, Tsutomu Arakawa, and Masao Tokunaga. “Efficient Production of Trastuzumab Fab Antibody Fragments in Brevibacillus Choshinensis Expression System.” Protein Expression and Purification 150, no. May (2018): 109–18. https://doi.org/10.1016/j.pep.2018.05.013.
伊東祐二, 金山洋介, 林良雄. “親和性ペプチドを用いた部位特異的修飾法による抗体の高機能化技術.” 実験医学 11 (2018): 1854–58.

2017

Ito, Yuji. “網羅的配列解析による多様な候補抗体の迅速同定手法の開発伊東祐二 Development of a Rapid Identification Method for a Variety of Antibody Candidates Using High-Throughput Sequencing.” YAKUGAKU ZASSHI 137, no. 7 (2017): 823–30.
Enomoto-Okawa, Yurie, Yuka Maeda, Nozomi Harashima, Yumika Sugawara, Fumihiko Katagiri, Kentaro Hozumi, Kam Man Hui, Motoyoshi Nomizu, Yuji Ito, and Yamato Kikkawa. “An Anti-Human Lutheran Glycoprotein Phage Antibody Inhibits Cell Migration on Laminin-511: Epitope Mapping of the Antibody.” Edited by Mitchell Ho. PLOS ONE 12, no. 1 (January 6, 2017): e0167860. https://doi.org/10.1371/journal.pone.0167860.
Uematsu, Shuta, Taiki Midorikawa, Yuji Ito, and Masumi Taki. “Selection of Turning-on Fluorogenic Probe as Protein-Specific Detector Obtained via the 10BASEd-T.” In AIP Conference Proceedings, 1807:020028. AIP Publishing LLC , 2017. https://doi.org/10.1063/1.4974810.
Khan, Kamrul Hasan, Arisa Himeno, Shouhei Kosugi, Yosuke Nakashima, Abdur Rafique, Ayana Imamura, Takaaki Hatanaka, Dai Ichiro Kato, and Yuji Ito. “IgY-Binding Peptide Screened from a Random Peptide Library as a Ligand for IgY Purification.” Journal of Peptide Science 23, no. 10 (2017): 790–97. https://doi.org/10.1002/psc.3027.
Kishimoto, Satoshi, and Yuji Ito. “ラクダ科動物重鎖抗体由来の抗原結合ドメインVHHの医療技術への応用（Therapeutic Applications of Antigen Binding Domain VHH Derived from Heavy Chain Antibodies in Camedidae）.” MEDCHEM NEWS 27, no. 1 (2017): 264–70.

2016

伊東祐二. “ヒトIgG特異的化学修飾技術による機能性抗体医薬の創出.” 細胞 48, no. 4 (2016): 18 (172)-22 (176).
Taki, Masumi, Hiroaki Inoue, Kazuto Mochizuki, Jay Yang, and Yuji Ito. “Selection of Color-Changing and Intensity-Increasing Fluorogenic Probe as Protein-Specific Indicator Obtained via the 10BASE d -T.” Analytical Chemistry 88, no. 2 (2016): 1096–99. https://doi.org/10.1021/acs.analchem.5b04687.
瀧真清, and 伊東祐二. “人工コアのファージ上での分子進化.” 生物工学会誌 94, no. 8 (2016): 473–76.
Muguruma, Kyohei, Fumika Yakushiji, Ryosuke Kawamata, Daichi Akiyama, Risako Arima, Takuya Shirasaka, Yamato Kikkawa, et al. “Novel Hybrid Compound of a Plinabulin Prodrug with an IgG Binding Peptide for Generating a Tumor Selective Noncovalent-Type Antibody–Drug Conjugate.” Bioconjugate Chemistry 27, no. 7 (2016): 1606–13. https://doi.org/10.1021/acs.bioconjchem.6b00149.
Nakayama, Hiroshi, Noriko Kenjjou, Nobuyuki Shigetoh, and Yuji Ito. “Fluorescence Immunoassay for Cocaine Detection.” Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 35, no. 2 (2016): 83–85. https://doi.org/10.1089/mab.2015.0067.
Nakayama, Hiroshi, Noriko Kenjyou, and Yuji Ito. “Development and Characterization of Monoclonal Antibodies Specific for 3-(1-Naphthoyl) Indole Derivatives.” Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 35, no. 1 (2016): 48–51. https://doi.org/10.1089/mab.2015.0045.
Abe, Yoshito, Mitsuru Kubota, Shinya Takazaki, Yuji Ito, Hiromi Yamamoto, Dongchon Kang, Tadashi Ueda, and Taiji Imoto. “Effect on Catalysis by Replacement of Catalytic Residue from Hen Egg White Lysozyme to Venerupis Philippinarum Lysozyme*.” Protein Science 25, no. 9 (2016): 1637–47. https://doi.org/10.1002/pro.2966.

2015

Mizukami, Makoto, Hiroko Tokunaga, Hiromasa Onishi, Yohei Ueno, Hiroshi Hanagata, Nobuo Miyazaki, Norihiko Kiyose, et al. “Highly Efficient Production of VHH Antibody Fragments in Brevibacillus Choshinensis Expression System.” Protein Expression and Purification 105 (2015): 23–32. https://doi.org/10.1016/J.PEP.2014.09.017.
橋口周平, 宮原隆二, 岸本聡, and 伊東祐二. “分子標的素子デザインにおけるファージライブラリー法.” 生物工学会誌 93, no. 5 (2015): 289–92. https://www.sbj.or.jp/wp-content/uploads/file/sbj/9305/9305_yomoyama.pdf.

2014

Muraoka, Junko, Takuya Ozawa, Yurie Enomoto, Norihiko Kiyose, Ayana Imamura, Kazunari Arima, Hiroshi Nakayama, and Yuji Ito. “Selection and Characterization of Human Serum Albumin-Specific Porcine ScFv Antibodies Using a Phage Display Library.” Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 33, no. 1: 42–48. https://doi.org/10.1089/mab.2013.0068.
Fukunaga, Keisuke, Takaaki Hatanaka, Yuji Ito, Michiko Minami, and Masumi Taki. “Construction of a Crown Ether-like Supramolecular Library by Conjugation of Genetically-Encoded Peptide Linkers Displayed on Bacteriophage T7.” Chem. Commun. 50, no. 30 (2014): 3921–23. https://doi.org/10.1039/C4CC00811A.
Tokunaga, Yuuki, Yuuki Azetsu, Keisuke Fukunaga, Takaaki Hatanaka, Yuji Ito, and Masumi Taki. “Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7.” Molecules 19, no. 2 (2014): 2481–96. https://doi.org/10.3390/molecules19022481.

2013

Muraoka, Jin, Noriho Kamiya, and Yuji Ito. “Preparation and Evaluation of Cellulose-Dissolving Magnetic Ionic Liquid.” Journal of Molecular Liquids 182 (2013): 76–78. https://doi.org/10.1016/J.MOLLIQ.2013.03.012.
Fukunaga, Keisuke, Takaaki Hatanaka, Yuji Ito, and Masumi Taki. “Gp10 Based-Thioetherification (10BASEd-T) on a Displaying Library Peptide of Bacteriophage T7.” Molecular BioSystems 9, no. 12 (2013): 2988. https://doi.org/10.1039/c3mb70379g.

2012

Hatanaka, Takaaki, Shinji Ohzono, Mirae Park, Kotaro Sakamoto, Shogo Tsukamoto, Ryohei Sugita, Hiroyuki Ishitobi, et al. “Human IgA-Binding Peptides Selected from Random Peptide Libraries: Affinity Maturation and Application in IgA Purification.” The Journal of Biological Chemistry 287, no. 51 (2012): 43126–36. https://doi.org/10.1074/jbc.M112.389742.